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Restore Western Blot Stripping Buffer

 

Thermo Scientific Restore Western Blot Stripping Buffer safely and effectively removes primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be re-probed.

 

Highlights:

  • Saves time – no need to re-run gels and blots

  • Saves costly sample – re-probe the membrane using the same target sample

  • Effective – formulation is more efficient at stripping antibodies than homemade buffers

  • Gentle – does not damage the target antigen during stripping allowing efficient reprobing

  • Odor-free – no mercaptans means no acrid odorsEconomical – less expensive than other commercial stripping buffers

Restore Plus Western Blot Stripping Buffer

 

Thermo Scientific Restore PLUS Western Blot Stripping Buffer is an advanced formula for removing bound primary and secondary antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

 

Highlights:

  • Ready and easy to use – no dilution necessary; no offensive odors; store at room temperature

  • Compatible – use on nitrocellulose and PVDF membranes, whether still wet or already dry; works with practically any blocking buffer, enzyme conjugate and chemiluminescent substrate

  • Cost effective – save valuable time and samples; strip blots effectively the first time

  • Robust yet gentle – transferred proteins remain viable; strip the same blot up to five times

  • Flexible – strip and reprobe to optimize antibody concentrations or to detect a new antigen with different antibodies