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Lane Marker Sample Buffers

 

Thermo Scientific Pierce Lane Marker Sample Buffers are ready-to-use 5X SDS-PAGE sample loading buffers, available in reducing and non-reducing formulations, with a pink dye buffer-front marker.

 

Highlights:

  • Easy to see – bright pink dye marks the buffer front, clearly indicating the progress of the electrophoresis run

  • Concentrated – 5X stocks enable a greater range of protein sample volumes to be used for electrophoresis

  • Transferable marker dye – pink dye transfers with protein to nitrocellulose membrane, enabling verification of transfer direction and orientation of the blot

  • Flexibility in SDS-PAGE applications – available in two ready-to-use formulations for reducing or non-reducing denaturing SDS polyacrylamide electrophoresis

LDS Sample Buffer, Non-Reducing (4X)

 

Thermo Scientific Pierce LDS Sample Loading Buffer (4X) is a non-reducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes. It is used in denaturing gels and is compatible with both coomassie and silver staining and western blotting applications.

 

Highlights:

  • Concentrated – 4X formulation provides more versatility than traditional 2X buffers

  • Non-reducing – ready to use for non-reducing SDS-PAGE

  • SDS alternative – lithium dodecyl sulfate (LDS) replaces sodium dodecyl sulfate (SDS) of traditional loading buffers but functions equally well

  • Physiologic pH – buffered with triethanolamine (pH 7.6) rather than Tris (pH 6.8)

  • Lane marker dyes – includes trace amounts of phenol red and coomassie G250 as visible markers of the dye front

  • Convenient – stable at room temperature, enabling storage at the bench where electrophoresis is performed