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Gel-Casting and Polymerization Reagents

Ammonium Persulfate (APS)

 

Thermo Scientific Pierce Ammonium Persulfate (APS) is an oxidizing agent that is used with TEMED to catalyze the polymerization of acrylamide and bisacrylamide to prepare polyacrylamide gels for electrophoresis. Ammonium persulfate (APS) is an oxidizing agent that is often used with tetramethylethylenediamine (TEMED, Part No. 17919) to catalyze the polymerization of acrylamide and bisacrylamide to prepare polyacrylamide gels for electrophoresis.

 

Highlights:

  • Name: Ammonium persulfate (APS)

  • Formula: (NH4)2S2O8CAS number: 7727-54-0

  • Molecular weight: 228.2

  • Purity: ≥98.0%

  • Insolubles: ≤0.005%

Tetramethylethylenediamine (TEMED)

 

Thermo Scientific Pierce Tetramethylethylenediamine (TEMED) is an essential catalyst for polyacrylamide gel polymerization. TEMED is used with ammonium persulfate (APS) to catalyze acrylamide polymerization when preparing gels for electrophoresis. The chemical is a liquid at room temperature.

 

Highlights:

  • Chemical name: N,N,N',N'-tetramethylethane-1,2-diamine

  • Formula: C6H16N2CAS number: 110-18-9

  • Molecular weight: 116.21

  • Purity: ≥99%

  • Refractive index: 1.417 to 1.419

  • Boiling range: 119 to 121°C