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BupH Modified Dulbecco's PBS Packs

 

Thermo Scientific BupH Dry-Blend Buffer Packs are pouches of pre-blended powder mixtures that are each sufficient to make 500mL buffer for protein sample preparation, storage and analysis methods. BupH Dry-Blend Buffer Packs are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve.

 

The packs eliminate weighing time and tedious pH adjustments.Modified Dulbecco's PBS Packs – Each pack makes 500mL of physiological Na- and K-phosphate buffered saline (8mM sodium phosphate, 2mM potassium phosphate, 0.14M NaCl, 10mM KCl, pH 7.4) for ELISA and Western blot diluents and wash buffers.

BuPH Phosphate Buffered Saline Packs

 

Each pack makes 500mL of sodium phosphate buffer (0.1M sodium phosphate, 0.15M NaCl, pH 7.2) for use in crosslinking and labeling reactions requiring amine-free conditions.

2BuPH Tris Buffered Saline Packs

 

Each pack makes 500mL of Tris-NaCl buffer (25mM Tris, 0.15M NaCl, pH 7.2) for diluents and wash buffers in ELISA, Western blotting and general immunoassay procedures.

BuPH Tris-Glycine Transfer Buffer Packs

 

Each pack makes 500mL of standard electrophoresis running buffer (25mM Tris, 192mM glycine, pH 8) for wet or semi-dry protein transfer from gel to blotting membrane.

BuPH Tris-Glycine-SDS Buffer Packs

 

Each pack makes 500mL of standard running buffer (25mM Tris, 192mM glycine, 0.1% SDS, pH 8.3) for SDS-PAGE (protein polyacrylamide gel electrophoresis)

Modified Dulbecco's PBS Tween-20, 20X

 

Thermo Scientific Pierce 20X Modified Dulbecco's PBS Tween-20 is used to prepare physiological Na- and K-phosphate buffered saline (D-PBS) as wash and blocking buffers for ELISA, western and other immunoassays. It makes 8mM sodium phosphate, 2mM potassium phosphate, 0.14M NaCl, 10mM KCl, 0.05% Tween™ 20, pH 7.4, when diluted to 1X with water.

Modified Dulbecco’s PBS, 20X

 

Thermo Scientific Pierce 20X Modified Dulbecco's PBS is used for preparing physiological Na- and K-phosphate buffered saline (D-PBS) as antibody diluents used in ELISA, western blotting and other immunoassays. It makes 8mM sodium phosphate, 2mM potassium phosphate, 0.14M NaCl, 10mM KCl, and pH 7.4, when diluted to 1X with water.

PBS Tween-20, 20X

 

Thermo Scientific Pierce 20X PBS Tween-20 is used as wash buffers for ELISA, western and other immunoassays as well as blocking buffer for plate-based assays. It makes 10mM sodium phosphate, 0.15M NaCl, pH 7.5, when diluted to 1X with water.

Phosphate Buffered Saline, 20X

 

Thermo Scientific Pierce 20X Phosphate Buffered Saline is used in crosslinking, biotinylation and fluorescent labeling reactions requiring an amine-free buffer. It makes 10mM sodium phosphate, 0.15M NaCl, pH 7.5, when diluted to 1X with water.

TBS Tween-20, 20X

 

Thermo Scientific Pierce 20X TBS Tween-20 is used as wash buffers or blocking buffer diluent used for ELISA, Western blotting and other immunoassays. It makes makes 25mM Tris, 0.15M NaCl, 0.05% Tween-20, pH 7.5, when diluted to 1X with water.

Tris Buffered Saline, 20X

 

Thermo Scientific Pierce 20X Tris Buffered Saline is used as wash buffer and antibody diluent for ELISA, Western blotting and other immunoassays. It makes 25mM Tris, 0.15M NaCl, pH 7.2 to 7.5, when diluted to 1X with water.

Tris-Glycine Buffer, 10X

 

Thermo Scientific Pierce 10X Tris-Glycine Buffer is ideal for quickly preparing standard Tris-glycine (pH 8.5) transfer buffer used for Western blotting. It makes 0.025M Tris, 0.192M glycine, pH 8.5, when diluted to 1X with water or 20% methanol.

Tris-Glycine-SDS Buffer, 10X

 

Thermo Scientific Pierce 10X Tris-Glycine-SDS Buffer is ideal for quickly preparing standard Tris-glycine-SDS (pH 8.3) running buffer used for SDS-PAGE (protein polyacrylamide gel electrophoresis). It makes 0.025M Tris, 0.192M glycine, 0.1% SDS, pH 8.5, when diluted to 1X with water.

Western Blot Transfer Buffer, 10X, Methanol-free

 

Thermo Scientific Pierce 10X Western Blot Transfer Buffer, Methanol-free is used for preparing the methanol-free transfer buffer used with cooled, tank (wet) western blotting transfer apparatus. It does not contain methanol and the addition of methanol is not necessary for use in electrophoresis.