Tel (Main): +65 - 6264 4628 | Fax: +65 - 6264 4625

IEF100 First-dimension Isoelectric Focusing Unit

 

Monitor current flow for each individual IPG strip in real time

 

The IEF100 is the only first dimension instrument that controls the current and voltage applied to the IPG strips to prevent overheating.

 

  • Flexible first-dimension IEF can be run with up to six 7 to 24 cm IPG strips simultaneously, or twelve 7 cm IPG strips using the included dual electrode accessory

  • Turn and click intuitive user interface with graphical display

  • Capable of faster run times with an integrated 12,000 V power supply that has the highest voltage and current commercially available

  • Easily accessible Ethernet and RS232 ports

SERVA IPG BlueStrips

 

Precast immobilized pH gradient IPG gel strips eliminate gradient drift and batch variability problems

 

SERVA IPG BlueStrips are low percentage, ultra thin, immobilized pH gradient gels cast onto a plastic backing support. They are used for the isoelectric focusing of protein samples and provide high resolution protein separations.

 

The strips can be used for rehydration or cup loading of proteins and can be used in all commercially available isoelectric focusing devices including the IEF100. The strips are available in five pH ranges and three strip lengths.

Carrier Ampholytes

 

SERVALYT™ carrier ampholytes are low molecular weight molecules of zwitterionic character. They are derived synthetically and comprise a multitude of varying pI-values. In agarose and polyacrylamide gels containing ampholytes, a linear pH-gradient will be built up when an electric field is applied-the ampholyte molecules carry a net charge and thus migrate in the electric field between the electrodes until they reach the position of corresponding pI. At this point they carry a net 0 charge, stop moving and form small plateaus (stationary stacks).

 

To achieve good separation of protein bands by IEF (isoelectric focusing), stable pH-gradients with extensive and consistent buffer capacity is required. SERVALYT carrier ampholytes yield reliable results and excellent reproducibility.

 

SERVALYT carrier ampholytes can be used for preparation of tube or slab isoelectric focusing gels (IEF), in free flow or preparative devices, or for rehydration of dried gels cast on film supports (such as immobilized pH strips or rehydratable slab gels). Without denaturants and reductants, proteins will be focused by their native pI (Isoelectric point). When denaturants and reductants are present, proteins will be focused in their denatured, unfolded, and reduced state. Carrier ampholytes frequently are included during sample preparation as they can also aid in protein solubility. Standard protocols typically use concentrations or 0.5%-2% carrier ampholyte in the gel and up to 4% in sample preparation.

 

Select SERVALYT carrier ampholytes based on the desired pH range or that which most closely matches the immobilized gel strip being used.

SE900 Large Format Vertical Gel Electrophoresis Unit

 

For highly reproducible second-dimension separations on up to six 28 cm SDS-PAGE gels

 

The SE900 was designed as a dedicated unit for second dimension electrophoretic separation. It can accommodate up to six 28 cm SDS-PAGE gels which mirrors the throughput of the IEF100 (6 IPG strips). If desired, the unit will accommodate plates of similar size made by other manufacturers.

 

  • No upper buffer chamber – no gasket issues and no risk of buffer loss

  • Utilizes minimal parts – easy to use and reliable

  • External cooling and internal buffer circulation – ensures an even, constant, and uniform temperature for reproducible results

  • Up to six 28 cm plates can be run at one time – perfect for high throughput applications

  • Glass plate sandwiches are hinged cassettes – allows for rapid and easy assembly before use

  • Built-in draining port – avoids lifting of a heavy buffer-filled tank

  • Assembly of the tank is easy – no clamps needed

  • Innovative design enables re-use of tank buffer – decreased buffer consumption for reduced cost

Mini 2-D Combination Package

 

Mini 2-D Electrophoresis System

 

The SE900 was designed as a dedicated unit for second dimension electrophoretic separation. It can accommodate up to six 28 cm SDS-PAGE gels which mirrors the throughput of the IEF100 (6 IPG strips). If desired, the unit will accommodate plates of similar size made by other manufacturers.

 

Includes:

  • IEF100 Isoelectric Focusing Unit

  • SE260-10A-.75 Mighty Small II Mini Deluxe Vertical Unit

  • SE2619T-2-1.0 T-Spacers, 1.0 mm Thick (qty. 2)

  • PS300B 300 V, 500 mA, 90 W Power Supply

Standard 2-D Combination Package

 

Standard 2-D Electrophoresis System

 

Includes:

  • IEF100 Isoelectric Focusing Unit

  • SE600X-15-1.5 Deluxe Dual Cooled Vertical Unit

  • PS300B 300 V, 500 mA, 90 W Power Supply

Large 2-D Combination Package

 

Large 2-D Electrophoresis System

 

Includes:

  • IEF100 Isoelectric Focusing Unit

  • SE900 Large Format Vertical Electrophoresis Unit

  • SE9102-1-1.0 Large Hinged Glass Cassettes (qty. 6)

  • SE915 Large Multiple Gel Caster

Large 2-D Combination Package w/P.S.

 

Voltage Requirement*

 

Includes:

  • IEF100 Isoelectric Focusing Unit

  • SE900 Large Format Vertical Electrophoresis Unit

  • SE9102-1-1.0 Large Hinged Glass Cassettes (qty. 6)

  • SE915 Large Multiple Gel Caster

  • PS600 Power Supply - 600 V, 750 mA, 150 W