Life Science

Solutions for Protein Biochemistry & Proteomics

Cell Lysis and Protein Extraction

 

Halt Phosphatase Inhibitor Cocktail

Thermo Scientific Halt Phosphatase Inhibitor Cocktail preserves the phosphorylation state of proteins during and after cell lysis or tissue protein extraction.

 

The Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from serine/threonine phosphatases as well as protein tyrosine phosphatases (PTPs) during cell lysis. The cocktail contains a mixture of four inhibitors of broad specificity, including sodium fluoride, sodium orthovanadate, sodium pyrophosphate and beta-glycerophosphate.

 

Conveniently packaged in a 100X format, the cocktail is stable at 4°C and is suitable for use with any cell or tissue extract. The cocktail preserves protein phosphorylation in immunoprecipitation and kinase assays.

 

Furthermore, the cocktail is compatible with popular protein assays such as the Thermo Scientific Pierce BCA Protein Assay Kit (Part No. 23225) and Bradford-based Pierce Coomassie Plus Assay Kit (Part No. 23236).